In vitro culture of rainbow trout, Oncorhynchus mykiss, gonads during sex differentiation and gonadogenesis

Guillevic M., Mourot B., Guiguen Y., Fostier A.

Date de parution: juillet 2008
Volume: 32
Number: 2 suppl.
Pagination: 107-108
Editeur: Société Française d'Ichtyologie

The histological features, steroidogenic activities (aromatase, 11b-hydroxylase) and gene expressions (cyp19A1, cyp11b1, amh, ddx4, gcl, fst) of trout embryonic gonads have been compared before and after 7 days of in vitro culture at 12°C in three different media. MCDB 105 gave the best results and was used to analyse the effect of 50 nM 17b-estradiol on testicular cyp11b1 expression. A significant six-fold decrease has been observed for cyp11b1, while amh, ddx4 and gcl did not change.

Mots-clés: Embryonic gonad - Estradiol - In vitro culture - Rainbow trout - Sexual differentiation
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