Sperm chromatin fragmentation and oxidization during short- and long-term storage of sex reversed rainbow trout (Oncorhynchus mykiss) males

Pérez-Cerezales S., Martínez-Páramo S., Herráez M.P.

Date de parution: juillet 2008
Volume: 32
Number: 2 suppl.
Pagination: 221
Editeur: Société Française d'Ichtyologie
doi: https://doi.org/10.26028/cybium/2008-322SP-105

Sperm storage is a basic tool for reproductive biotechnology that causes cell damage at different levels. Our objective was to detect DNA strand breaks or nucleotide oxidization promoted by short and long-term storage in spermatozoa from sex-reversed rainbow trout, using the Comet assay and previous digestion with specific endonucleases. Short-term storage promotes strand breaks and oxidization, especially in guanines. Cryopreservation increases DNA fragmentation, but apparently does not cause oxidization, suggesting possible apoptotic events.

Mots-clés: Comet assay - Cryopreservation - DNA damage - Sperm - Trout
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