Estrogenic activity of nonylphenol in marine fish, Hexagrammos otakii, during oocyte development by evaluating sex steroid levels

Hwang I.J., Lee Y.D., Kim H.B., Baek H.J.

Date de parution: juillet 2008
Volume: 32
Number: 2 suppl.
Pagination: 251-252
Editeur: Société Française d'Ichtyologie

In this study, we examined the potential estrogenic activity of nonylphenol (NP) on oocyte development of a marine fish, Hexagrammos otakii, using steroid hormone assays. Vitellogenic (0.80-82, 0.92-96 and 1.08-12 mm diameter) and fully matured (1.90-95 mm diameter) oocytes were in vitro exposed to NP at different concentrations (0.1, 1, 10 and 100 ng/ml) with or without 50IU human chorionic gonadotropin (HCG). NP increased the estradiol-17β (E2)/testosterone (T) ratio at 0.1, 1 and 10 ng/ml in the oocytes of 0.80-82 and 0.92-96 mm. In the oocytes of 1.08-12 mm, which were fully vitellogenic stage, NP increased E2/T ratio only at 100 ng/ml. In the oocytes of 1.90-95 mm, NP increased E2/17α,20β- dihydroxy-4-pregnen-3-one (17α20βP) ratio at all concentrations tested without HCG and decreased E2/17α20βP ratio in the presence of HCG but not in a dose-dependent manner. Taken together, these results suggest that NP exhibits estrogenic activity through ovarian development of H. otakii.

Mots-clés: Estrogenic activity - Nonylphenol - Oocyte development - Steroid
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