Effects of cortisol and 11-ketotestosterone (11-KT) on testicular development and sperm quality in male spotted wolffish
Sperm production and characteristics of male spotted wolffish (Anarhichas minor) treated with cortisol, 11-KT, or a combination of both steroids, were compared with sham treated controls. Cortisol treatment significantly reduced proportions of sperm producing males and tended to reduce seminal fluid pH. 11-KT significantly increased proportions of motile sperm cells and gave increased fertilisation potential. There were strong negative correlations between [Na+] and [Mg2+] (R2 = 0.91), and between [Na+] and [Ca2+] (R2 = 0.86) in the seminal fluid. Further, [Mg2+] and [Ca2+] tended to be higher and [Na+] lower in cortisol-treated fish (alone or in combination with 11-KT) than in controls and fish treated with 11-KT. The results indicate that steroids have an influence on sperm production and seminal fluid characteristics.