Biological characteristics of European hake (Merluccius merluccius) sperm

Groison A.L., Suquet M., Cosson J., Le Coz J.R., Jolivet A., Garren F.

Date de parution: juillet 2008
Volume: 32
Number: 2 suppl.
Pagination: 178
Editeur: Société Française d'Ichtyologie

Very little is known on European hake’s reproductive biology and especially on biological characteristics of its sperm despite a growing interest in its aquaculture potential. This study reports, for the first time some hake sperm characteristics. After activation, the swimming phase lasts 3 min (8 min when activated with 50% sea water (SW) but lower initial velocity). The initial flagellar beat frequency (BF), velocity and percentage of motile cells decreased after 100 s. After 2 days at 4°C, the mean Adenylate Energy Charge (AEC) level was 0.71. Sperm stored at 4°C still showed motility after 9 days for 2 individuals. When cryopreserved, the motility recovery index of thawed spermatozoa ranged from 11.8% to 29.6%.

Mots-clés: AEC - Gamete quality - Merluccius merluccius - Sperm Density - Sperm motility
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