Pituitary gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play central roles in regulating gametogenesis and the production of gonadal hormones, in fish as in other vertebrates. Pituitary gonadotropins are composed of two non-covalently associated polypeptide subunits, which must be glycosylated, folded, and assembled as a heterodimer to be biologically active. Since biotechnology for the production of recombinant proteins has been greatly advanced, it is possible that fish FSH and LH are produced biotechnologically as an alternative way for various manipulations in modern aquaculture. This review discusses different platforms currently being applied for recombinant piscine gonadotropin production.