Expression of nitric oxide synthase isoforms in the ovary of Heteropneustes fossilis (Bloch.) during follicular development and oocyte maturation
The aim of the study was to demonstrate the presence of nitric oxide synthase (NOS) isoforms and role of nitric oxide (NO) in ovary of H. fossilis. The immunohistochemistry (IHC) showed eNOS and iNOS in granulosa and theca cells and nNOS in ooplasm of early previtellogenic follicles. Western blot confirmed the presence of three types of NOS in ovary. The ovarian concentrations of nitric oxide were significantly higher in pre-vitellogenic than vitellogenic phase. The in vitro study showed the inhibitory action of NO in oocytes maturation. The results suggest a role of NO in follicular development in H. fossilis.