A new species, Diancistrus typhlops (Ophidiiformes, Bythitidae), is described from six adult specimens (51-80 SL) caught in the dark zone in three anchialine caves on Muna Island, southeast of Sulawesi. It differs from the 27 species of Diancistrus hitherto described by being eyeless and by the combination of the number of dorsal fin rays and length of head. Based on the pseudoclasper morphology, the most similar species is Diancistrus leisi Schwarzhans, Møller & Nielsen, 2005, caught in the Coral Sea and on the Great Barrier Reef. The new species differs from D. leisi by the numbers of dorsal fin rays 78-81 vs 69-73 and anal fin rays 63-65 vs 55-57, and by lacking pseudobranchial filaments vs two filaments.