Ecuador, located on the central-eastern edge of the Pacific Ocean, has a high level of biological diversity, including no less than 800 species of marine fish. The ecological and economic importance, as well as the vulnerability of cartilaginous fish along the coasts of Ecuador and the Galapagos Islands, justify the need to produce an updated list of this group. Marine fish are generally listed separately and no updated national list has been produced recently. The objective of this work is to present an updated list of the continental and insular chondrichthyans of Ecuador based on field observations in the fishing ports of Santa Rosa de Salinas (Santa Elena province), Puerto López and Manta (Manabí province), alongside a review of the specialized literature. This group of fish includes 12 orders, 39 families, 65 genera, and 119 species. Of these, 64 are sharks, 48 are rays, and 7 are chimaeras.