Knipowitschia radovici sp. nov. is described from the Neretva drainage basin, Dalmatia, Croatia. The new species is assigned to the Gobiidae Knipowitschia based on diagnostic features belonging to the genus. The new species differs from congeners by the reduction of cephalic lateral line canals, the reduction in squamation and the arrangement of some sensory papillae rows. Morphological characters of Knipowitschia species are discussed.