For a quantitative examination of the accumulation by growing oocytes of the multiple yolk proteins which are derived from three forms of vitellogenins in barfin flounder, Verasper moseri, six enzyme-linked immunosorbent assays (ELI SAs) were developed using antisera against vitellogenin (Vg) A, VgB, VgC as phosvitinless vitellogenin, lipovitellin (Lv) A, LvB and LvC, respectively. Concentrations of the Vgs in serum and liver, and contents of the Lvs in ovarian follicles were measured by the developed ELISAs in female fish sampled throughout the year. Results of the measurements show that this controlled accumulation of multiple Vgs is regulated primarily by rates of hepatic Vg synthesis and secretion and secondarily by mechanisms for receptor-mediated uptake of Vgs into oocytes.